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Description

van Geest, F

The assembled RAGRagulator complex was eluted using wash buffer with 250 mM imidazole and further purified by SEC with a Superdex 200 10/300 GL column

Similarly, cultured cells were collected in 40 L of RIPA buffer per well (of a six-well plate) and placed on ice for 1 h, centrifuged and the supernatant transferred to a new tube for protein content and WB analysis